CD26/DPP4 Cell-Surface Expression in Bat Cells Correlates with Bat Cell Susceptibility to Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection and Evolution of Persistent Infection
Identifieur interne : 001D28 ( Main/Exploration ); précédent : 001D27; suivant : 001D29CD26/DPP4 Cell-Surface Expression in Bat Cells Correlates with Bat Cell Susceptibility to Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection and Evolution of Persistent Infection
Auteurs : Yíngyún Caì [États-Unis] ; Shu Qìng Yú [États-Unis] ; Elena N. Postnikova [États-Unis] ; Steven Mazur [États-Unis] ; John G. Bernbaum [États-Unis] ; Robin Burk [États-Unis] ; Téngf I Zh Ng [États-Unis] ; Sheli R. Radoshitzky [États-Unis] ; Marcel A. Müller [Allemagne] ; Ingo Jordan [Allemagne] ; Laura Bollinger [États-Unis] ; Lisa E. Hensley [États-Unis] ; Peter B. Jahrling [États-Unis] ; Jens H. Kuhn [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux (immunologie), Cellules Vero, Chiroptera (virologie), Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Dipeptidyl peptidase 4 (métabolisme), Humains, Infections à coronavirus (transmission), Infections à coronavirus (virologie), Lignée cellulaire, Récepteurs viraux (métabolisme), Réservoirs d'agents pathogènes (virologie), Susceptibilité à une maladie, Tropisme viral.
- MESH :
- immunologie : Anticorps monoclonaux.
- métabolisme : Dipeptidyl peptidase 4, Récepteurs viraux.
- pathogénicité : Coronavirus du syndrome respiratoire du Moyen-Orient.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- virologie : Chiroptera, Infections à coronavirus, Réservoirs d'agents pathogènes.
- transmission : Animaux, Cellules Vero, Humains, Infections à coronavirus, Lignée cellulaire, Susceptibilité à une maladie, Tropisme viral.
English descriptors
- KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Cell Line, Chiroptera (virology), Chlorocebus aethiops, Coronavirus Infections (transmission), Coronavirus Infections (virology), Dipeptidyl Peptidase 4 (metabolism), Disease Reservoirs (virology), Disease Susceptibility, Humans, Middle East Respiratory Syndrome Coronavirus (pathogenicity), Middle East Respiratory Syndrome Coronavirus (physiology), Receptors, Virus (metabolism), Vero Cells, Viral Tropism.
- MESH :
- chemical , immunology : Antibodies, Monoclonal.
- chemical , metabolism : Dipeptidyl Peptidase 4, Receptors, Virus.
- pathogenicity : Middle East Respiratory Syndrome Coronavirus.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- transmission : Coronavirus Infections.
- virology : Chiroptera, Coronavirus Infections, Disease Reservoirs.
- Animals, Cell Line, Chlorocebus aethiops, Disease Susceptibility, Humans, Vero Cells, Viral Tropism.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) is a recently isolated betacoronavirus identified as the etiologic agent of a frequently fatal disease in Western Asia, Middle East respiratory syndrome. Attempts to identify the natural reservoirs of MERS-CoV have focused in part on dromedaries. Bats are also suspected to be reservoirs based on frequent detection of other betacoronaviruses in these mammals. For this study, ten distinct cell lines derived from bats of divergent species were exposed to MERS-CoV. Plaque assays, immunofluorescence assays, and transmission electron microscopy confirmed that six bat cell lines can be productively infected. We found that the susceptibility or resistance of these bat cell lines directly correlates with the presence or absence of cell surface-expressed CD26/DPP4, the functional human receptor for MERS-CoV. Human anti-CD26/DPP4 antibodies inhibited infection of susceptible bat cells in a dose-dependent manner. Overexpression of human CD26/DPP4 receptor conferred MERS-CoV susceptibility to resistant bat cell lines. Finally, sequential passage of MERS-CoV in permissive bat cells established persistent infection with concomitant downregulation of CD26/DPP4 surface expression. Together, these results imply that bats indeed could be among the MERS-CoV host spectrum, and that cellular restriction of MERS-CoV is determined by CD26/DPP4 expression rather than by downstream restriction factors.
Url:
DOI: 10.1371/journal.pone.0112060
PubMed: 25409519
PubMed Central: 4237331
Affiliations:
- Allemagne, États-Unis
- Berlin, District de Cologne, Maryland, Rhénanie-du-Nord-Westphalie
- Berlin, Bonn
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Le document en format XML
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Postnikova</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Mazur, Steven" sort="Mazur, Steven" uniqKey="Mazur S" first="Steven" last="Mazur">Steven Mazur</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Bernbaum, John G" sort="Bernbaum, John G" uniqKey="Bernbaum J" first="John G." last="Bernbaum">John G. Bernbaum</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Burk, Robin" sort="Burk, Robin" uniqKey="Burk R" first="Robin" last="Burk">Robin Burk</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Zh Ng, Tengf I" sort="Zh Ng, Tengf I" uniqKey="Zh Ng T" first="Téngf I" last="Zh Ng">Téngf I Zh Ng</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Radoshitzky, Sheli R" sort="Radoshitzky, Sheli R" uniqKey="Radoshitzky S" first="Sheli R." last="Radoshitzky">Sheli R. Radoshitzky</name><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A." last="Müller">Marcel A. Müller</name><affiliation wicri:level="3"><nlm:aff id="aff3"><addr-line>Institute of Virology, University of Bonn Medical Centre, Bonn, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Virology, University of Bonn Medical Centre, Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Jordan, Ingo" sort="Jordan, Ingo" uniqKey="Jordan I" first="Ingo" last="Jordan">Ingo Jordan</name><affiliation wicri:level="3"><nlm:aff id="aff4"><addr-line>ProBioGen AG, Berlin, Germany</addr-line></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>ProBioGen AG, Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Bollinger, Laura" sort="Bollinger, Laura" uniqKey="Bollinger L" first="Laura" last="Bollinger">Laura Bollinger</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E." last="Hensley">Lisa E. Hensley</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B." last="Jahrling">Peter B. Jahrling</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H." last="Kuhn">Jens H. Kuhn</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Integrated Research Facility at Fort Detrick, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Fort Detrick, Frederick, Maryland</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Cell Line</term><term>Chiroptera (virology)</term><term>Chlorocebus aethiops</term><term>Coronavirus Infections (transmission)</term><term>Coronavirus Infections (virology)</term><term>Dipeptidyl Peptidase 4 (metabolism)</term><term>Disease Reservoirs (virology)</term><term>Disease Susceptibility</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (pathogenicity)</term><term>Middle East Respiratory Syndrome Coronavirus (physiology)</term><term>Receptors, Virus (metabolism)</term><term>Vero Cells</term><term>Viral Tropism</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Cellules Vero</term><term>Chiroptera (virologie)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (pathogénicité)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term><term>Dipeptidyl peptidase 4 (métabolisme)</term><term>Humains</term><term>Infections à coronavirus (transmission)</term><term>Infections à coronavirus (virologie)</term><term>Lignée cellulaire</term><term>Récepteurs viraux (métabolisme)</term><term>Réservoirs d'agents pathogènes (virologie)</term><term>Susceptibilité à une maladie</term><term>Tropisme viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Dipeptidyl Peptidase 4</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dipeptidyl peptidase 4</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Infections à coronavirus</term><term>Réservoirs d'agents pathogènes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Coronavirus Infections</term><term>Disease Reservoirs</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Disease Susceptibility</term><term>Humans</term><term>Vero Cells</term><term>Viral Tropism</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Humains</term><term>Infections à coronavirus</term><term>Lignée cellulaire</term><term>Susceptibilité à une maladie</term><term>Tropisme viral</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Middle East respiratory syndrome coronavirus (MERS-CoV) is a recently isolated betacoronavirus identified as the etiologic agent of a frequently fatal disease in Western Asia, Middle East respiratory syndrome. Attempts to identify the natural reservoirs of MERS-CoV have focused in part on dromedaries. Bats are also suspected to be reservoirs based on frequent detection of other betacoronaviruses in these mammals. For this study, ten distinct cell lines derived from bats of divergent species were exposed to MERS-CoV. Plaque assays, immunofluorescence assays, and transmission electron microscopy confirmed that six bat cell lines can be productively infected. We found that the susceptibility or resistance of these bat cell lines directly correlates with the presence or absence of cell surface-expressed CD26/DPP4, the functional human receptor for MERS-CoV. Human anti-CD26/DPP4 antibodies inhibited infection of susceptible bat cells in a dose-dependent manner. Overexpression of human CD26/DPP4 receptor conferred MERS-CoV susceptibility to resistant bat cell lines. Finally, sequential passage of MERS-CoV in permissive bat cells established persistent infection with concomitant downregulation of CD26/DPP4 surface expression. Together, these results imply that bats indeed could be among the MERS-CoV host spectrum, and that cellular restriction of MERS-CoV is determined by CD26/DPP4 expression rather than by downstream restriction factors.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Groot, Rj" uniqKey="De Groot R">RJ de Groot</name></author><author><name sortKey="Baker, Sc" uniqKey="Baker S">SC Baker</name></author><author><name sortKey="Baric, Rs" uniqKey="Baric R">RS 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sortKey="Cai, Yingyun" sort="Cai, Yingyun" uniqKey="Cai Y" first="Yíngyún" last="Caì">Yíngyún Caì</name></region><name sortKey="Bernbaum, John G" sort="Bernbaum, John G" uniqKey="Bernbaum J" first="John G." last="Bernbaum">John G. Bernbaum</name><name sortKey="Bollinger, Laura" sort="Bollinger, Laura" uniqKey="Bollinger L" first="Laura" last="Bollinger">Laura Bollinger</name><name sortKey="Burk, Robin" sort="Burk, Robin" uniqKey="Burk R" first="Robin" last="Burk">Robin Burk</name><name sortKey="Hensley, Lisa E" sort="Hensley, Lisa E" uniqKey="Hensley L" first="Lisa E." last="Hensley">Lisa E. Hensley</name><name sortKey="Jahrling, Peter B" sort="Jahrling, Peter B" uniqKey="Jahrling P" first="Peter B." last="Jahrling">Peter B. Jahrling</name><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H." last="Kuhn">Jens H. Kuhn</name><name sortKey="Mazur, Steven" sort="Mazur, Steven" uniqKey="Mazur S" first="Steven" last="Mazur">Steven Mazur</name><name sortKey="Postnikova, Elena N" sort="Postnikova, Elena N" uniqKey="Postnikova E" first="Elena N." last="Postnikova">Elena N. Postnikova</name><name sortKey="Radoshitzky, Sheli R" sort="Radoshitzky, Sheli R" uniqKey="Radoshitzky S" first="Sheli R." last="Radoshitzky">Sheli R. Radoshitzky</name><name sortKey="Yu, Shu Qing" sort="Yu, Shu Qing" uniqKey="Yu S" first="Shu Qìng" last="Yú">Shu Qìng Yú</name><name sortKey="Zh Ng, Tengf I" sort="Zh Ng, Tengf I" uniqKey="Zh Ng T" first="Téngf I" last="Zh Ng">Téngf I Zh Ng</name></country><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Muller, Marcel A" sort="Muller, Marcel A" uniqKey="Muller M" first="Marcel A." last="Müller">Marcel A. Müller</name></region><name sortKey="Jordan, Ingo" sort="Jordan, Ingo" uniqKey="Jordan I" first="Ingo" last="Jordan">Ingo Jordan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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